What you will learn in Working with DNA

1. 1. Cutting DNA

   Finding the pattern in four recognition sequences before being told it.

2. 2. Why each cloning step exists

   A working plasmid, and three ways to break it diagnosably.

3. 3. PCR

   Exponential amplification, and two ways to get nothing from it.

4. 4. Sizing a fragment

   Reading an unknown against a ladder, on a logarithmic scale.

5. 5. Recombinant insulin

   Six stages, two of which a bacterium cannot do.

6. 6. Separating the questions

   Which claims about biotechnology evidence can settle, and which it cannot.